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tryple® select enzyme (10×), no phenol red  (Thermo Fisher)


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    Structured Review

    Thermo Fisher tryple® select enzyme (10×), no phenol red
    Tryple® Select Enzyme (10×), No Phenol Red, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tryple+select+enzyme+10+%C3%97+phenol+red/tryple+select+enzyme/us12084683-193-40-42
    Average 90 stars, based on 1 article reviews
    tryple® select enzyme (10×), no phenol red - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Protocol to develop a 3D mouse autologous lung tumor-immune spheroid co-culture model to advance radiotherapy-based treatments
    Article Snippet: TrypLE Select enzyme (10×), no phenol red , Gibco , Ref: A1217701.

    Article Title: Development of an In Vitro Human Thyroid Microtissue Model for Chemical Screening
    Article Snippet: The cells were counted using the trypan blue exclusion method on a Countess automated cytometer (ThermoFisher Scientific, Waltham, Massachusetts).

    Centrifugation:

    Article Title: Robust and reproducible human intestinal organoid-derived monolayer model for analyzing drug absorption.
    Article Snippet: .. After centrifugation at 200 × g for 5 min at 4 °C and the removal of supernatant, 2 mL of 2.5 × TrypLE Select solution (mixture of TrypLE select (10 ×) (Thermo Fisher Scientific):PBS ( −) = 1:3) was added to the conical tube, and organoid fragments were digested into single cells in a 37 °C water bath for 5–10 min. Then, 2 mL of basal medium was added to each conical tube, and the mixture was well-pipetted and passed through a 70 μm cell strainer. ..

    Cell Culture:

    Article Title: Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation
    Article Snippet: .. As pretreatment, Ff-hPSC-derived aggregates were cultured in 20 μM Y-27632 for 24 h. The pretreated aggregates were dissociated into single cells by treatment with neuron dissociation solution (Wako) at 37°C for 30 min; repeated washing with DMEM/F-12 (Thermo Fisher Scientific); treatment with DMEM/F-12 containing 0.2% collagenase type I (Wako) and 0.1% BSA at 37°C for 30 min with gentle stirring; repeated washing with PBS; treatment with a 10× TrypLE Select solution (Thermo Fisher Scientific) containing 0.2 mg/mL DNase I (Roche) at 37°C for 10 min; and gentle separation by pipetting. .. The cells were filtered through a 70-μm cell strainer cap (BD Biosciences) and resuspended in sorting buffer (DMEM/F-12) containing 1 mM EDTA, 1% FBS, and 20 μM Y-27632.

    Article Title: Caco-2 cells as a model for intestinal absorption.
    Article Snippet: The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms.. Permeability values estimated with this model correlate well with human in vivo absorption data for many drugs and chemicals.. As a consequence, the use of the Caco-2 cellular model as a permeability assay to predict oral absorption in humans is growing, and its importance is increasing as a screening tool in drug-discovery strategies for the prediction of intestinal drug permeability.

    Gentle:

    Article Title: Generation and purification of ACTH-secreting hPSC-derived pituitary cells for effective transplantation
    Article Snippet: .. As pretreatment, Ff-hPSC-derived aggregates were cultured in 20 μM Y-27632 for 24 h. The pretreated aggregates were dissociated into single cells by treatment with neuron dissociation solution (Wako) at 37°C for 30 min; repeated washing with DMEM/F-12 (Thermo Fisher Scientific); treatment with DMEM/F-12 containing 0.2% collagenase type I (Wako) and 0.1% BSA at 37°C for 30 min with gentle stirring; repeated washing with PBS; treatment with a 10× TrypLE Select solution (Thermo Fisher Scientific) containing 0.2 mg/mL DNase I (Roche) at 37°C for 10 min; and gentle separation by pipetting. .. The cells were filtered through a 70-μm cell strainer cap (BD Biosciences) and resuspended in sorting buffer (DMEM/F-12) containing 1 mM EDTA, 1% FBS, and 20 μM Y-27632.

    Microscopy:

    Article Title: Caco-2 cells as a model for intestinal absorption.
    Article Snippet: The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms.. Permeability values estimated with this model correlate well with human in vivo absorption data for many drugs and chemicals.. As a consequence, the use of the Caco-2 cellular model as a permeability assay to predict oral absorption in humans is growing, and its importance is increasing as a screening tool in drug-discovery strategies for the prediction of intestinal drug permeability.

    Positron Emission Tomography:

    Article Title: Caco-2 cells as a model for intestinal absorption.
    Article Snippet: The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms.. Permeability values estimated with this model correlate well with human in vivo absorption data for many drugs and chemicals.. As a consequence, the use of the Caco-2 cellular model as a permeability assay to predict oral absorption in humans is growing, and its importance is increasing as a screening tool in drug-discovery strategies for the prediction of intestinal drug permeability.

    Membrane:

    Article Title: Caco-2 cells as a model for intestinal absorption.
    Article Snippet: The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms.. Permeability values estimated with this model correlate well with human in vivo absorption data for many drugs and chemicals.. As a consequence, the use of the Caco-2 cellular model as a permeability assay to predict oral absorption in humans is growing, and its importance is increasing as a screening tool in drug-discovery strategies for the prediction of intestinal drug permeability.

    Pore Size:

    Article Title: Caco-2 cells as a model for intestinal absorption.
    Article Snippet: The Caco-2 cell system, a well characterized intestinal in vitro model, makes it possible to evaluate the ability of chemicals to cross the intestinal barrier, as well as to study their transport mechanisms.. Permeability values estimated with this model correlate well with human in vivo absorption data for many drugs and chemicals.. As a consequence, the use of the Caco-2 cellular model as a permeability assay to predict oral absorption in humans is growing, and its importance is increasing as a screening tool in drug-discovery strategies for the prediction of intestinal drug permeability.



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